Menu
Close
Sign up for NPL updates
Close
Sign up for NPL updates

Receive regular emails from NPL to get a glimpse of our activities and see how our experts are informing and influencing scientific debate

Biometrology measurements

Protein production and characterisation

Production of high-quality protein samples

Proteins have many important functions and are widely used for industrial, nutritional and medical applications. Production of recombinant proteins is an important way to generate large amounts of a specific protein, and is typically achieved by the manipulation of gene expression in an organism so that it expresses large amounts of a recombinant gene.

Production of recombinant proteins is crucial for functional activity studies, structure determination and development of biopharmaceutical products. Our state-of-the-art protein production and characterisation platform offers the following measurements services:

  • Protein design and engineering
  • Expression of soluble and membrane proteins in E. coli (T7 based expression vector systems), insect Cells (Sf9, Sf21 and Hi-5) or yeast (S. cerevisiae)
  • Purification of soluble and membrane proteins
  • Membrane protein detergent screen using F-SEC approaches
  • Incorporation of membrane proteins in nanodiscs
  • Protein characterisation using SEC-MALS, high throughput and time-resolved DLS measurements (2μL per sample, up to 70 samples in parallel) or CD measurements

Our services can be tailored according to exact customer needs. The key equipment supporting this service includes:

  • HPLC purifying systems (AKTA)
  • Automated 4C SEC-MALS system (Malven)
  • Automated high throughput F-SEC system (Shimadzu)
  • Automated high throughput DLS system (SpectroLight 620/XtalConcepts)
  • UV-Vis Spectrophotometer NanoDrop One (Labtech)
  • Gel imaging system + ChemiDoc MP (BioRad)

Don’t see what you are looking for? Our diverse skill set enables us to provide bespoke solutions. Please contact us to discuss your requirements.

Contact us

Research

We develop cutting edge measurement tools and methods to unlock new applications, from drug discovery to synthetic biology. Our knowledge of biophysical measurements helps us understand the relationship between biological systems and processes.

Work with us

Our research and measurement solutions support innovation and product development. We work with companies to deliver business advantage and commercial success.
Contact our Customer Services team on +44 20 8943 7070