NPL is the UK's National Metrology Institute, developing and maintaining the national primary measurement standards. The National Centre of Excellent in Mass Spectrometry Imaging (NiCE-MSI) at NPL and collaborators introduced OrbiSIMS in 2017 for 3D chemical imaging of molecules and it has become increasingly popular for biological and material sciences owing to its ability to give simultaneous high confidence in molecular identity and localisation.
With a growing number of instruments internationally, there is an increased need for reproducible measurements. In recent works we realised that Signal, Noise and Transmission form a metrology triangle that can provide a secure foundation and lead to innovative analytical methods and new technologies.
OrbiSIMS is done on a hybrid instrument with a dual analyser instrument combining a Time-of-Flight (ToF) mass spectrometer (MS) and an OrbitrapTM MS, which confer advantages of speed and high-performance mass spectrometry, respectively. The VAMAS TWA 2 A37 MS interlaboratory comparison aims to build on the methods previously published and study reproducibility of OrbiSIMS experiments for organic and inorganic materials.
Benefits for participating in the intercomparison:
Noise characteristics measured
Conversion factor of Orbitrap intensity to counts
Signal linearity of instrument
Reproducibility of relative and absolute signal