Sauerbrey Mass adsorbed on the surface calculated from QCMD data (left)
Comparison between Ellipsometric and XPS thickness of different adsorbed proteins (right)

Accurate quantification of adsorbed biomolecules is important in the design of new biomaterials, diagnostic devices and sensors, as well as for the understanding of biomolecular interactions on surfaces. There are many methods for measuring the amount of biomolecules attached to surfaces, for example, ellipsometry, X-ray photoelectron spectroscopy (XPS), surface plasmon resonance (SPR) and quartz crystal microbalance (QCM). However, the accuracy of these techniques and their comparability has not been adequately explored, and our initial work in this area is addressing this issue. The efficiency of bio-conjugation methods on the concentration, structure and activity of biomolecules is a future aim of our work.

Selected Publications

[1] Preparation and characterisation of ethylenediamine and cysteamine plasma polymerised films on piezoelectric quartz crystal surfaces for a biosensor, S Mutlu, D Cokelilar, A Shard, H Goktas, B Ozansoy, M Mutlu, Thin Solid Films 516 (2008) 1249

[2] Biocompatibility and the efficacy of medical implants, A G Shard, P E Tomlins, Regenerative Medicine 1 (2006) 789

If you require PDF copies of the publications, please contact us below.

Contact: Santanu Ray or Alex Shard