STORM-ing through the diffraction limit
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Beating the Diffraction Limit:
- Instead of having all the fluorescent molecules on, switch most of them off.
- Measure the middle position of each fluorescent molecule and plot its position.
- Switch a different subset of the fluorescent molecules on and repeat steps 1 and 2 until all of them have been plotted. Typically this is done on 10000 or more frames.
- Put together a super-resolution image using all the plotted molecular positions from the previous steps. Each plotted position is called a localisation and is represented by a superresolution pixel. The brighter a pixel is the more localisations there are within that area.
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