Beating the Diffraction Limit:

(1) Instead of having all the fluorescent molecules on, switch most of them off.

(2) Measure the middle position of each fluorescent molecule and plot its position.

(3) Switch a different subset of the fluorescent molecules on and repeat steps 1 and 2 until all of them have been plotted. Typically this is done on 10000 or more frames.

(4) Put together a super-resolution image using all the plotted molecular positions from the previous steps. Each plotted position is called a localisation and is represented by a superresolution pixel. The brighter a pixel is the more localisations there are within that area.

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