National Physical Laboratory

STORM-ing through the diffraction limit

Further Information

Published: 22 May 2012

Author: D Metcalf

Related: Biotechnology

(PDF 141 KB)



Beating the Diffraction Limit:

  1. Instead of having all the fluorescent molecules on, switch most of them off.
  2. Measure the middle position of each fluorescent molecule and plot its position.
  3. Switch a different subset of the fluorescent molecules on and repeat steps 1 and 2 until all of them have been plotted. Typically this is done on 10000 or more frames.
  4. Put together a super-resolution image using all the plotted molecular positions from the previous steps. Each plotted position is called a localisation and is represented by a superresolution pixel. The brighter a pixel is the more localisations there are within that area.

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Last Updated: 24 Sep 2014
Created: 22 May 2012


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