National Physical Laboratory

Josephine Bunch

Current interests

Dr Josephine Bunch

Josephine's interests include:

  • Mass spectrometry instrument and method development
  • Fundamentals of MALDI MS (matrix assisted laser desorption/ionisation mass spectrometry) and ambient mass spectrometry techniques
  • Measurement standardisation and data processing tools for mass spectrometry imaging
  • Application of mass spectrometry imaging in life sciences and drug discovery


Professor Josephine Bunch is a Principal Scientist and Co-Director of the National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI) at NPL and Chair of Biomolecular Mass Spectrometry at Imperial College London. At NPL, Josephine leads research and metrology in MALDI and ambient mass spectrometry imaging within NiCE-MSI. She is currently leading a Cancer Research UK Grand Challenge programme. Josephine previously led a research group in mass spectrometry imaging at the University of Birmingham, where she was a Lecturer in Chemistry and Imaging in the School of Chemistry and PSIBS Doctoral Training Centre (2009–2013).

Josephine completed a PhD in mass spectrometry imaging at Sheffield Hallam University, sponsored by Pfizer Global R&D (2005). She moved to the University of Sheffield (Department of Chemistry) in 2004 for her postdoctoral appointments (2004–2009). During this time, she was awarded an independent Enterprise Fellowship to support commercialisation of mass spectrometry imaging activities.

Josephine has expertise in a range of mass spectrometry imaging techniques and has published 55 articles on mass spectrometry imaging of lipids, drugs, proteins, peptides and metabolites using a range of dedicated mass spectrometry techniques. Josephine's group comprises a multidisciplinary team of physicists, chemists, computer scientists and biomedical scientists. The group host PhD students from the University of Nottingham, the University of Birmingham, Imperial College London, Oxford University and the University of Surrey.

Current projects are funded by Cancer Research UK, the National Measurement System, NC3Rs, EPSRC and Innovate UK, and UK plc.

Find out more about the Cancer Research UK Grand Challenge project

In 2019, Josephine was appointed as an NPL Fellow in the area of Biomolecular Analysis.

Selected publications

  • Analysis of urine, oral fluid and fingerprints by liquid extraction surface analysis coupled to high resolution MS and MS/MS – opportunities for forensic and biomedical science
    Bailey, M., Randall, E.C., Costa, C., Salter, T. L., Race, A.M. de Puit, M., Koeberg, M., Baumert, M. and Bunch J.
    Analytical Methods, 2016
  • Investigating MALDI MSI parameters (Part 1) – A systematic survey of the effects of repetition rates up to 20 kHz in continuous raster mode
    Rory T. Steven, Alex Dexter and Josephine Bunch
    Methods, 2016
  • Liquid Extraction Surface Analysis Mass Spectrometry Coupled with Field Asymmetric Waveform Ion Mobility Spectrometry for Analysis of Intact Proteins from Biological Substrates
    Sarsby, J., Griffiths, R. L., Race, A. M., Bunch,J., Randall, E. C., Creese, A. J., and Cooper, H. J.
    Analytical Chemistry, 87 (13), pp 6794–6800, 2015
  • Single-cell Analysis: Visualizing Pharmaceutical and Metabolite Uptake in Cells with Label-free 3D Mass Spectrometry Imaging
    Passarelli, M. K., Newman, C. J., Marshall, P. S., West, A., Gilmore, I.S., Bunch, J., Alexander, M.R., Dollery, C.T.
    Analytical Chemistry, 2015
  • Optimisation of colour schemes to accurately display mass spectrometry imaging data based on human colour perception
    Race, A. M., and Bunch, J.
    Analytical and Bioanalytical Chemistry, 407 (8), pp 2047–2054, 2015
Last Updated: 1 Feb 2019
Created: 6 Nov 2013


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