National Physical Laboratory

Jascindra Ravi

Current interests

Jascindra Ravi

Jascindra Ravi joined NPL to work on protein structure analysis after completing her PhD. During the first couple of years, she spent her time validating circular dichroism (CD) for protein structure analysis and was involved in the development of a CD standard. This was followed by a period developing FTIR as an early detection tool for methionine oxidation in bio-pharmaceuticals.

Currently, she is working on the development and application of measurement methods to advance the understanding of protein structure, assembly and function relationships at biomolecular interfaces. This involves the study of interactions between peptides such as antimicrobial peptides (AMP), cell penetrating peptides (CPP), HIV peptides and amyloid β peptides with artificial membranes using biophysical techniques.

Biography

After completing an MSc in Bioanalytical Chemistry (Instrumental methods in biological and Environmental (pharmaceutical) Chemistry) at the University of Warwick, Jascindra stayed on to complete a PhD in Biophysical Chemistry. Her research involved structural characterisation of macromolecular interactions in the solution phase using polarized spectroscopic techniques such as circular and linear dichroism. Within the research programme, new applications for flow linear dichroism including protein-membrane interactions and single walled carbon nanotube-DNA interactions were explored. Finally the potential of the new technique, infrared flow linear dichroism, was investigated with DNA.

Selected publications

  • Membrane mediated regulation in free peptides of HIV-1 gp41: Minimal modulation of the hemifusion phase
    E. Cerasoli, J. Ravi, C. Gregor, R. Hussain, G. Siligardi, G. Martyna, J. Crain and M. G. Ryadnov
    Physical Chemistry Chemical Physics (2011)
    DOI: 10. 1039/b000000x
  • FTIR markers of methionine oxidation for early detection of oxidized protein therapeutics
    J. Ravi, A. E. Hills, E. Cerasoli, P. D. Rakowska, M. G. Ryadnov
    European Biophysics Journal, 40, pp 339-345 (2011)
    PMID: 21229353
  • GeT peptides: a single-domain approach to gene delivery
    B. Lamarre, J. Ravi, M. G. Ryadnov
    Chemical Communications, 47 (32), pp 9045-9047 (2011)
    DOI: 10.1039/C1CC13043A
  • International comparability in spectroscopic measurements of protein structure by circular dichroism: CCQM-P59.1
    J. Ravi, P. D. Rakowska, T. Garfagnini, B. Baron, P. Charlet, C. Jones, J. DeSa Lorenz, D. Plusquellic, F. Wien, L. Wu, C. W. Meuse, A. E. Knight
    Metrologia, 47, pp 631-641 (2010)
    DOI: 10.1088/0026-1394/47/6/001
  • A new reference material for UV-visible circular dichroism spectroscopy
    A. Damianoglou, E. J. Crust, M. R. Hicks, S. E. Howson, A. E. Knight, J. Ravi, P. Scott, A. Rodger
    Chirality, 20 (9), pp 1029-1038 (2008)
    PMID: 18506835

Contact details

E-mail: jascindra.ravi@npl.co.uk
Tel: 020 8943 6035

Last Updated: 23 Jun 2014
Created: 5 Dec 2011

Registration

Please note that the information will not be divulged to third parties, or used without your permission

Login