Current Interests

Daniel Metcalf has an interest in the development of fluorescence microscopy approaches and their use in studying neurodegeneration. He is currently involved in the development of super-resolution microscopy systems - this includes the preparation of cell samples and identifying novel biological problems for super-resolution approaches.

Biography

Daniel completed his PhD in Molecular Cell Biology at University College London in 2008. He then became a post-doctoral researcher at the Institute of Neurology (University College London) where he was involved in cell biology research of frontotemporal dementia and the development of ahigh-throughput confocal assay for assessing the autophagy pathway. Subsequently, Daniel had a short post-doctoral position at Cambridge University, where he again studied autophagy pathways linked to the cell biology of neurodegeneration. He has published in several molecular and cell biology journals, including a FRAP-based confocal method for assessing intracellular trafficking of fluorescent proteins from the Golgi.

Selected Publications

• Loss of the Batten disease gene CLN3 prevents exit from the TGN of the mannose 6-phosphate receptor
  D. J. Metcalf, A. A. Calvi, M. N. J. Seaman, H. Mitchison, D. F. Cutler
  Traffic, 9 (11), pages 1905-14 (2008)
  PMID: 18817525
• Disruption of endocytic trafficking in frontotemporal dementia with CHMP2B mutations
  H. Urwin, A. Authier, J. E. Nielsen, D. Metcalf, et al
  Hum Mol Genet, 19 (11), pages 2228-38 (2010)
  DOI: 10.1093/hmg/ddq100
• The role of ESCRT proteins in fusion events involving lysosomes, endosomes and autophagosomes
  D. Metcalf, A. M. Isaacs
  Biochem Soc Trans, 38 (6), pages 1469-73 (2010)
  DOI: 10.1042/BST0381469
• Autophagy and misfolded proteins in neurodegeneration
  D. J. Metcalf, M. Garcia-Arencibia, D. C. Hochfeld, Rubinsztein
  Exp Neurol. (2010)
  PMID: 21095248

Contact details

E-mail: daniel.metcalf@npl.co.uk
Tel: 020 8943 6344