National Physical Laboratory

Lead verification using SPR and ITC technologies

Lead verification using SPR and ITC technologies 

  • The bio-analytical group at NPL is able to carry out analysis of many macromolecular interactions and provide lead verification for antibody and small molecule candidates.

  • We can provide a rapid screening service using our state of the art Biacore T100 SPR instrument, which will provide a qualitative ranking of the best binders from a panel of candidates based on single cycle kinetics.

  • We can also verify any leads generated by the initial screen and provide high resolution multi-cycle kinetics for standard antibody-ligand, antibody-receptor systems and small molecule-enzyme interactions. The on rate (ka) and off rate (kd) as well as the equilibrium dissociation constant (KD) are critical parameters for the selection of the most promising candidates and the Biacore T100 instrument is recognised as the industry leader for determining these parameters.

We can also measure the thermodynamics of interactions between any two biomolecules using a technique called Isothermal Titration Calorimetry (ITC). This technique is considered the 'gold standard' for the measurement of the binding affinity of a given biomolecular interaction and is able to determine the enthalpy (ΔH), entropy (ΔS) of binding and the stoichiometry of the interaction. This technique does not require the binding partners to be labeled or either of them to be immobilised on a surface. For this reason the instrument measures the interaction under true, in-solution, equilibrium conditions. 

Using ITC NPL can also help companies carry out structure activity relationship (SAR) studies, where the analysis of the changes in ΔH and ΔS can help reveal the mechanism of binding for small molecules and fragments.

If you are interested in any of these services, please contact us.

Contact

Customer Service tel: +44 20 8943 8637
E-mail: bioanalytical_enquiries@npl.co.uk

Last Updated: 28 Oct 2014
Created: 6 May 2009