National Physical Laboratory

BiotechnologyPublications

Biotechnology Group publications since 2011.

Representative Publications


Binary encoding of random peptide sequences for selective and differential antimicrobial mechanisms
Hayouka, Z. et al
Angew Chem Int Ed, 2017, 56, 8099–8103

Engineering monolayer poration for rapid exfoliation of microbial membranes
Pyne, A. et al
Chem Sci, 2017, 8, 1105–1115

Autonomously folded α-helical lockers promote RNAi
Guyader, C. et al
Sci Rep, 2016, 6, 35012

A de novo virus-like topology for synthetic virions
Noble, J. et al
J Am Chem Soc, 2016, 138, 12202–12210

Nano-mechanical single-cell sensing of cell-matrix contacts
Zajiczek, L. et al
Nanoscale, 2016, 8, 18105–18112

Structurally plastic peptide capsules for synthetic antimicrobial viruses
Castelletto, V. et al
Chem Sci, 2016, 7, 1707–1711

Role of liposome and peptide in the synergistic enhancement of transfection with a lipopolyplex vector
Munye, M. et al
Sci Rep, 2015, 19, 9292

Exploitable length correlations in peptide nanofibres
De Santis, E. et al
Nanoscale, 2014, 6, 11425–11430

Filming protein fibrillogenesis in real time
Bella, A. et al
Sci Rep, 2014, 4, 7529

Differentially instructive extracellular protein micro-nets
Faruqui, N. et al
J Am Chem Soc, 2014, 136, 7889–7898

Cicada-inspired cell-instructive nanopatterned arrays
Diu, T. et al
Sci Rep, 2014, 4, 7122

Nanoscale imaging reveals laterally expanding antimicrobial pores in lipid bilayers
Rakowska, P. et al
Proc Natl Acad Sci USA, 2013, 110, 8918–8923

Anti-antimicrobial peptides: coiled-coil-mediated host defense antagonism
Ryan L., et al
J Biol Chem, 2013, 288, 20162–20172

Arbitrary self-assembly of peptide extracellular microscopic matrices
Bella A. et al
Angew Chem Int Ed, 2012, 51, 428–431

2017


Binary encoding of random peptide sequences for selective and differential antimicrobial mechanisms
Hayouka, Z., Bella, A., Stern, T., Ray, S., Jiang, H., Grovenor, C. R. M. & Ryadnov, M. G.
Angew Chem Int Ed, 2017, 56, 8099–8103

Linear and orthogonal peptide templating of silicified protein fibres
Bella, A., Ray, S. & Ryadnov, M. G.
Org Biomol Chem, 2017, 15, 5380–5385.

Protein-inspired synthetic biology: new forms for old functions
Ryadnov, M. G.
Reference Module in Chem Mol Sci Chem Eng (Comprehensive Supramol Chem II), 2017, 439–458

Modulating charge-dependent and folding-mediated antimicrobial interactions at peptide-lipid interfaces
Iavicoli, P., Rossi, F., Lamarre, B., Bella, A., Ryadnov, M. G. and Calzolai, L.
Eur Biophys J, 2017, 46, 375–382

Nano-mechanical in-process monitoring of antimicrobial poration in model phospholipid bilayers
Valsesia, A., Iavicoli, P., Lewis, H., Desmet, C., Mehn, D., Calzolai, L., Colpo, P., Rossi, F. & Ryadnov, M. G.
RSC Adv, 2017, 7, 19081–19084

In-vivo high resolution AFM topographic imaging of Caenorhabditis elegans reveals previously unreported surface structures of cuticle mutants
Essmann, C. L., Elmi, M., Shaw, M., Anand, G. M., Pawar, V. M. and Srinivasan, M. A.
Nanomedicine, 2017, 13, 183–189

Engineering monolayer poration for rapid exfoliation of microbial membranes
Pyne, A., Pfeil, M.-P., Bennett, I., Ravi, J., Iavicoli, P., Lamarre, B., Roethke, A., Ray, S., Jiang, H., Bella, A., Reisinger, B., Yin, D., Little, B., Muñoz-García, J. C., Cerasoli, E., Judge, P. J., Faruqui, N., Calzolai, L., Henrion, A., Martyna, G. J., Grovenor, C. R. M., Crain, J., Hoogenboom, B. W., Watts, A. and Ryadnov, M. G
Chem Sci, 2017, 8, 1105–1115

2016 - 2011


Biophysical modulation of peptide-membrane interactions
Ravi, J. and Ryadnov, M. G.
Amino Acids, Pept. Proteins, 2016, 41, 188-224

Nano-mechanical single-cell sensing of cell-matrix contacts
Zajiczek, L., Shaw, M., Faruqui, N., Bella, A., Pawar, V. M., Srinivasan, M. A. and Ryadnov, M. G.
Nanoscale, 2016, 8, 18105-18112

Autonomously folded α-helical lockers promote RNAi
Guyader, C. P. E., Lamarre, B., De Santis, E., Noble, J. N., Slater, N. K. and Ryadnov, M. G.
Sci Rep, 2016, 6, 35012; doi: 10.1038/srep35012

A de novo virus-like topology for synthetic virions
Noble, J. E., De Santis, E., Ravi, J., Lamarre, B., Castelletto, V., Mantell, J., Ray, S. and Ryadnov, M. G.
J Am Chem Soc, 2016, 138, 12202-12210

Amino acids, peptides and proteins
Ryadnov, M. G and Hudecz, F.
Amino Acids, Pept. Proteins, 2016, 41, 272

Investigation of mechanosensation in C. elegans using light field calcium imaging
Shaw, M., Elmi, M., Pawar, V. and Srinivasan, M. A.
Biomed Opt Express, 2016, 7, 2877-2887

Two pillars of fledgling RNA medicine
Ryadnov, M. G.
Eur. Biopharm. Rev., 2016, 3, 46-48

What is the 'Minimum Inhibitory Concentration' (MIC) of pexiganan acting on Escherichia coli? – a cautionary case study
Jepson, A. K., Schwarz-Linek, J., Ryan, L., Ryadnov, M. G. & Poon, W. C.
Adv Exp Med Biol, 2016, 915, 33-48

Epithelial-mesenchymal transition, IP3 receptors and ER-PM junctions: translocation of Ca2+ signalling complexes and regulation of migration
Okeke, E., Parker, T., Dingsdale, H., Concannon, M., Awais, M., Voronina, S., Molgó, J., Begg, M., Metcalf, D., Knight, A.E., Sutton, R., Haynes, L., and Tepikin A.V.
Biochem. J., 2016, 473, 757-767

Super-resolution microscopy as a potential approach to diagnosis of platelet granule disorders
Westmoreland, D., Shaw, M., Grimes, W., Metcalf, D. J., Burden, J. J., Gomez, K., Knight, A.E. and Cutler, D.F.
J. Thromb. Haemost., 2016, 13, 1-11

Nanoparticle metrology of silica colloids and super-resolution studies using the ADOTA fluorophore
Stewart, H.L., Yip, P., Rosenberg, M., Just Sørensen, T., Laursen, B.W., Knight A.E. and Birch, D.J.S.
Meas. Sci. Technol., 2016, 27, 045007

Structurally plastic peptide capsules for synthetic antimicrobial viruses
Castelletto, V., De Santis, E., Alkassem, H., Lamarre, B., Noble, J. E., Ray, S., Bella, A., Burns, J. R., Hoogenboom, B. W. and Ryadnov, M. G.
Chem Sci, 2016, 7, 1707-1711


Self-assembling peptide motifs for nanostructure design and applications
De Santis, E. and Ryadnov, M. G.
Amino Acids, Pept. Proteins, 2015, 40, 199-238

Characterization of antibody conjugated particles and their influence on diagnostic assay response
Worsley, G. J., Kumarswami, N., Minelli, C. and Noble, J. E.
Anal Meth, 2015, 7, 9596-9603

Reference materials and representative test materials to develop nanoparticle characterization methods: the NanoChOp project case
Roebben, G., Kestens, V., Varga, Z., Charoud-Got, J., Ramaye, Y., Gollwitzer, C., Bartczak, D., Geißler, D., Noble, J., Mazoua, S., Meeus, N., Corbisier, P., Palmai, M., Mihály, J., Krumrey, M., Davies, J., Resch-Genger, U., Kumarswami, N., Minelli, C., Sikora, A. and Goenaga-Infante, H.
Front Chem, 3, 56, doi: 10.3389/fchem.2015.00056

Interfacial zippering-up of coiled-coil protein filaments
De Santis, E., Castelletto, V. and Ryadnov, M. G.
Phys Chem Chem Phys, 2015, 17, 31055-31060

Application of asymmetric flow field-flow fractionation hyphenations for liposome-antimicrobial peptide interaction
Iavicoli, P., Urban, P., Bella, A., Ryadnov, M. G., Rossi, F. and Calzolai, L.
J Chromatogr A, 2015, 1422, 260-269

Amino acids, peptides and proteins
Ryadnov, M. G and Hudecz, F.
Amino Acids, Pept. Proteins, 2015, 40, 240

Recent innovations in super-resolution microscopy
Knight, A. and Peckham, M.
Methods, 2015, 88, 1-2

Super-resolution fluorescent methods: where next for super-resolution?
Knight, A., Kaminski, C. F. and Sauer, M.
Methods Appl. Fluoresc., 2015, 3, 030201

Peptide self-assembly for nanomaterials: the old new kid on the block
De Santis, E. and Ryadnov, M. G.
Chem. Soc. Rev, 2015, 44, 8288-8300

Supramolecular amphipathicity for probing antimicrobial propensity of host defence peptides
Ravi, J., Bella, A., Correia, A. J. V., Lamarre, B. and Ryadnov, M. G.
Phys Chem Chem Phys, 2015, 17, 15608-15614

High speed structured illumination microscopy in optically thick samples
Shaw, M., Zajiczek, L. and O'Holleran, K.
Methods, 2015, 88, 11-19

Role of liposome and peptide in the synergistic enhancement of transfection with a lipopolyplex vector
Munye, M. M., Ravi, J., Tagalakis, A. D., McCarthy, D., Ryadnov, M. G. and Hart, S. L.
Sci. Rep., 2015, 19, 9292, doi: 10.1038/srep09292

CCQM-P58.1: immunoassay quantitation of human cardiac troponin I
Bunk, D., Noble, J., Knight, A. E., Wang, L., Klauenberg, K., Walzel, M. and Elster, C.
Metrologia, 2015, 52, 08006, doi:10.1088/0026-1394/52/1A/08006

The elusive nature and diagnostics of misfolded Aβ oligomers
Cerasoli, E., Ryadnov, M. G. and Austen, B. M.
Front Chem., 2015, 3, 17, doi: 10.3389/fchem.2015.00017

Evaluation of standardization capability of current cardiac troponin I assays by a correlation study: results of an IFCC pilot project
Tate, J. R., Bunk, D. M., Christenson, R. H., Barth, J. H., Katrukha, A., Noble, J. E., Schimmel, H., Wang, L. and Panteghini, M.
Clin Chem Lab Med, 2015, 53, 677-690


Filming protein fibrillogenesis in real time
Bella, A., Shaw, M., Ray, S. and Ryadnov, M. G.
Sci. Rep., 2014, 4, 7529, doi: 10.1038/srep07529

Cicada-inspired cell-instructive nanopatterned arrays
Diu, T., Faruqui, N., Sjöström, T., Lamarre, B., Jenkinson, H. F., Su, B. and Ryadnov, M. G.
Sci. Rep., 2014, 4, 7122, doi: 10.1038/srep07122

Amino acids, peptides and proteins
Farkas, E. and Ryadnov, M. G.
Amino Acids, Pept. Proteins, RSC Publishing, 2014, 39, 236

Flat clathrin lattices: stable features of the plasma membrane
Grove, J., Metcalf, D. J., Knight, A. E., Wavre-Shapton, S. T., Sun, T., Protonotarios, E., Griffin, L. D., Lippincott-Schwartz, J. and Marsh, M.
Mol. Biol. Cell., 2014, 25, 3581-3594

Exploitable length correlations in peptide nanofibres
De Santis, E., Faruqui, N., Noble, J. E. and Ryadnov, M. G.
Nanoscale, 2014, 6, 11425-11430

Single-molecule fluorescence imaging by total internal reflection fluorescence microscopy
Knight, A. E.
Pure Appl Chem, 2014, 86, 1303-1320

Temperature denaturation and aggregation of a multi-domain protein (IgG1) investigated with an array of complementary biophysical methods
Cerasoli, E., Ravi, J., Garfagnini, T., Gnaniah, S., le Pevelen, D. and Tranter, G. E.
Anal Bioanal Chem, 2014, 406, 6577-6586

Optimized approaches for optical sectioning and resolution enhancement in structured illumination microscopy
O'Holleran, K., Shaw, M.
Biomed Opt Express, 2014, 5(8), 2580-2590

Recommendation to harmonize the units for reporting cardiac troponin results
Barth, J. H., Panteghini, M., Bunk, D. M., Christenson, R. H., Katrukha, A., Noble, J. E., Schimmel, H., Wang, L., Tate, J. R. for the IFCC Working Group on Standardization of Cardiac Troponin I.
Clin Chim Acta, 2014, 432, 166

Where is the drug gone? Measuring intracellular delivery and localization
Ryadnov, M. G.
Methods, 2014, 68, 281-282

Differentially instructive extracellular protein micro-nets
Faruqui, N., Bella, A., Ravi, J., Ray, S. and Ryadnov, M. G.
J Am. Chem. Soc., 2014, 136, 7889-7898

A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells
Ferraro, F., Kriston-Vizi, J., Metcalf, D. J., Martin-Martin, B., Freeman, J., Burden, J. J., Westmoreland, D., Dyer, C. E., Knight, A. E., Ketteler, R. and Cutler, D. F.
Dev Cell, 2014, 29, 292-304

TestSTORM: simulator for optimizing sample labeling and image acquisition in localization based super-resolution microscopy
Sinkó, J., Kákonyi, R., Rees, E., Metcalf, D., Knight, A. E., Kaminski, C. F., Szabó, G. and Erdélyi, M.
Biomed Opt Express 2014, 5, 778-787

Probing label-free intracellular quantification of free peptide by MALDI-ToF mass spectrometry
Rakowska, P. D., Lamarre, B. and Ryadnov, M. G.
Methods, 2014, 68, 331-337

Synthetic extracellular matrix biology
Ryadnov, M. G.
Synth Biol, 2014, 1, 187-229

Stable isotope imaging of biological samples with high resolution secondary ion mass spectrometry and complementary techniques
Jiang, H., Favaro, E., Goulbourne, C. N., Rakowska, P. D., Hughes, G. M., Ryadnov, M. G., Fong, L. G., Young, S. G., Ferguson, D. J., Harris, A. L. and Grovenor, C. R.
Methods, 2014, 68, 317-324

Synthetic biology
Ryadnov, M. G., Brunsveld, L. and Suga, H.
Synth Biol, 2014, 1, 300

Quantification of protein concentration using UV absorbance and coomassie dyes
Noble, J. E.
Methods Enzymol, 2014, 536, 17-26

Exploring the feasibility of human antimicrobial peptides for new and more efficient antibiotics
Ryadnov, M.
Drug Discov World, 2014, 15, 9-12

Uncertainty in measurement of protein circular dichroism spectra
Cox, M. G., Ravi, J., Rakowska, P. D. and Knight, A E.
Metrologia, 2014, 51, 67-79


Elements of image processing in localization microscopy
Eric J Rees, Miklos Erdelyi, Gabriele S Kaminski Schierle, Alex Knight and Clemens F Kaminski
Journal of Optics 15(9):094012

Test Samples for Optimizing STORM Super-Resolution Microscopy
Metcalf, D. ., Edwards, R., Kumarswami, N., Knight, A.
J. Vis. Exp. (79), e50579, doi:10.3791/50579 (2013)

Bio-functional peptide design
M. G. Ryadnov
Amino Acids, Pept. Proteins (Farkas and Ryadnov, eds.) RSC Publishing 2013, 38, 79-121

Peptidome analysis: tools and technologies
P. D. Rakowska & M. G. Ryadnov
Amino Acids, Pept. Proteins (Farkas and Ryadnov, eds.) RSC Publishing 2013, 38, 172-202

Amino Acids, Peptides and Proteins
E. Farkas & M. G. Ryadnov
Amino Acids, Pept. Proteins. RSC Publishing 2013, 38, 252

Investigation of the confocal wavefront sensor and its application to biological microscopy
Shaw M., O'Holleran K., Paterson C.
Opt. Express 2013, 21, 19339-19352

Anti-antimicrobial peptides: coiled-coil-mediated host defense antagonism
Ryan L., Lamarre B., Diu T., Ravi J., Judge P. J., Temple A., Carr M., Cerasoli E., Su B., Jenkinson H. F., Martyna G., Crain J., Watts A. & Ryadnov M. G.
J Biol. Chem. 2013, 288, 20162-20172

Nanoscale imaging reveals laterally expanding antimicrobial pores in lipid bilayers
Rakowska P.D., Jiang H., Ray S., Pyne A., Lamarre B., Carr M., Judge P. J., Ravi J, M Gerling U. I., Koksch B., Martyna G. J., Hoogenboom B. W. , Watts A. , Crain J., Grovenor C. R. & Ryadnov M. G.
Proc Natl Acad Sci U S A
2013, 110, 8918-8923

Biomimetic self-assembling nanoparticles
Ryadnov M.G.
Adv. Drug Deliv. 2013 (Mitra AK, Lee CH, Cheng K, eds.), Wiley-Blackwell, John Wiley & Sons, In

Three dimensional cell morphometry for the quantification of cell-substrate interactions
Shaw M., Faruqui N., Gurdak E. & Tomlins P.
Tissue Eng Part C Methods.
2013, 19, 48-56

Correcting chromatic offset in multicolor super-resolution localization microscopy
Erdelyi M., Rees E., Metcalf D., Kaminski Schierle, G. S., Dudas L., Sinko J., Knight A. E., & Kaminski C. F.
Opt Express
2013, 21, 10978-10988

Aptamer-mediated detection of thrombin using silver nanoparticle signal enhancement
Szymanski M., Noble J., Knight A. E., Porter R. & Worsley G.
Anal. Methods 2013, 5, 187-191


Collaborative research leads to new understanding of biomarkers.
Ryadnov M. G. & Crain J.
Drug Discov. World 2012, 13, 61-66.

Blind assessment of localisation microscope image resolution
Rees E. J., Erdelyi M., Pinotsi D., Knight A. E., Metcalf D., & Kaminski C. F.
Opt. Nanoscopy 2012, 1, 12-22.

Polarization effects on contrast in structured illumination microscopy
O'Holleran K. & Shaw M. J.
Opt. Lett., 2012, 37, 4603-4605.

Prescriptive peptide design
Ryadnov M. G.
Amino Acids, Pept. Proteins (Farkas, E., Ryadnov, M. G., eds.) RSC Publishing 2012, 37, 190-237

Amino Acids, Peptides and Proteins
Farkas E. & Ryadnov M. G.
Amino Acids, Pept. Proteins.
RSC Publishing 2012, 37, 1-270

Optical scattering artifacts observed in the development of multiplexed surface enhanced Raman spectroscopy. nanotag immunoassays
Noble J., Attree S., Horgan A., Knight A. E., Kumarswami N., Porter & Worsley G.
Anal. Chem. 2012, 84, 8246-8252

Rapid duplex immunoassay for wound biomarkers at the point-of-care.
Worsley G. J., Attree S. L., Noble J. E. & Horgan A. M.
Biosens. Bioelectron2012, 34, 215-220

Metallopolymer-peptide hybrid materials: synthesis and self-assembly of functional, polyferrocenylsilane-tetrapeptide conjugates
Tangbunsuk S., Whittell G. R., Ryadnov M. G., Vandermeulen G. W., Woolfson D. N. & Manners I.
Chem. Eur J, 2012, 18, 2524-2535

Natively unfolded state for engineering nanoscale fibrillar arrays
Ryadnov M. G. & Cherny D. I.
Macromol. Biosci. 2012, 12, 195-201

Membrane mediated regulation in free peptides of HIV-1 gp41: minimal modulation of the hemifusion phase
Cerasoli E., Ravi J., Gregor C. R., Hussain R., Siligardi G., Martyna G. J., Crain J. & Ryadnov M. G.
Phys. Chem. Chem. Phys. 2012, 14, 1277-1285

Arbitrary self-assembly of peptide extracellular microscopic matrices
Bella A., Ray S., Shaw M. & Ryadnov M. G.
Angew. Chem. Int. Ed. 2012, 51, 428-431


Random coil: a-helix equilibria as a reporter for the LewisX-LewisX interaction
Altamore T.M., Fernández-García C., Gordon A.H., Hübscher T., Promsawan N., Ryadnov M. G., Doig A. J., Woolfson D. N. & Gallagher T.
Angew. Chem. Int. Ed. 2011, 50, 11167-11171.

GeT peptides: a single-domain approach to gene delivery
Lamarre B., Ravi J. & Ryadnov M. G.
Chem. Comm., 2011, 47, 9045-9047.

Antibody Recognition of the epitope (hCG{beta}66-80) depends on local structure retained in the free peptide
Gregor C.R., Cerasoli E., Schouten J., Ravi J., Slootstra J., Horgan A., Martyna G. J., Ryadnov M. G., Davis P. & Crain J.
J. Biol. Chem. 2011 286, 25016-25026.

Bayesian analysis of an international ELISA comparability study
Klauenberg K., Ebert B., Voigt J., Walzel M., Noble J. E., Knight A. E. & Elster C.
Clin. Chem. Lab. Med. 2011, 49, 1459-68.

Silver nanoparticles and magnetic beads with electrochemical measurement as a platform for immunosensing devices
Szymanski M., Porter R., Dep G. V., Wang Y. &Haggett B. G. D.
Phys. Chem. Chem. Phys. 2011, 13, 5383-5387.

FTIR markers of methionine oxidation for early detection of oxidized protein therapeutics
Ravi J., Hills A. E., Cerasoli E., Rakowska P. D. & Ryadnov M. G.
Eur. Biophys. J. 2011, 40, 339-345.

Autonomous folding in the membrane proximal HIV peptide gp41659-671: pH tuneability at micelle interfaces
Gregor C. R., Cerasoli E., Tulip P. R., Ryadnov M. G., Martyna G. J. & Crain J.
Phys. Chem. Chem. Phys. 2011, 13, 127-135.

Chemical and biological characterisation of a sensor surface for bioprocess monitoring
Moore J. D., Perez-Pardo M. A., Popplewell J. F., Spencer S. J., Ray S., Swann M. J., Shard A. G., Jones W., Hills A. & Bracewell D. G.
Bios. Bioelectron. 2011, 26, 2940-2947.

Investigation of bovine serum albumin denaturation using ultrasonic spectroscopy
Povey M. J. W., Moore J. D., Braybrook J., Simons H., Belchamber R., Raganathan M. & Pinfield V.
Food Hydrocoll. 2011, 25, 1233-1241.

Viscosity of concentrated therapeutic protein composition
Jezek J., Rides M., Derham B., Moore J., Cerasoli E., Simler R. & Perez-Ramirez B.
Adv. Drug Deliv. Rev. 2011, 63, 1107-1117.

FT-IR markers of methionine oxidation
Ravi J. & Ryadnov M. G.
Bruker Optics Application Note AN#93 2011, BOPT-110613-1.

Single Enzyme Studies: A Historical Perspective
Knight A.
Single Molecule Enzymology; Methods Mol. Biol. 2011, 778, 1-9.

Nano-enabled biomarker discovery and detection
Rakowska P. D & Ryadnov M. G.
Biomark. Med. 2011, 5, 387-396.

Cellular uptake and intracellular fate of engineered nanoparticles: A review on the application of imaging techniques
Tantra R. & Knight A.
Nanotoxicology
2011, 778, 1-9.

The rational development of molecularly imprinted polymer-based sensors for protein detection
Whitcombe M. J , Chianella I., Larcombe L., Piletsky S. A., Noble J., Porter R. & Horgan A.
Chem. Soc. Rev. 2011, 40, 1547-1571.

Self-assembling viral mimetics: one long journey with short steps
Lamarre B. & Ryadnov M. G.
Macromol. Biosci., 2011, 11, 503-513.

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